The invention discloses a multi-source combined cold and heat supply simulated experiment greenhouse, which comprises a side wall, a roof, a meteorological station and a greenhouse environment regulating system, wherein the side wall and the roof are used for surrounding and forming a greenhouse internal space, the meteorological station is arranged outside the greenhouse, the greenhouse environment regulating system is used for regulating environment parameters of the greenhouse internal space, the greenhouse environment regulating system comprises heat consumption units, heat sources and a control unit, the heat sources supply heat to the heat consumption units, the control unit coordinates the energy transmission between the heat sources and the heat consumption units, the heat sourcesare a solar heat collector, a geothermy heat collector and a boiler, the solar heat collector, the geothermy heat collector and the boiler are respectively provided with water circulation pipelines, the water circulation pipelines of the solar heat collector, the geothermy heat collector and the boiler are connected into a heat collecting water tank in a parallel connection way, and the heat collecting water tank supplies heat to each heat consumption unit. The multi-source combined cold and heat supply simulated experiment greenhouse has the advantages that the combined cold and heat supply system realizing the conversion of various energy sources is built, the proper environment temperature is provided according to the growth requirements of crops cultured inside the greenhouse, the consumption of conventional energy sources can be greatly reduced, and the energy consumption optimization effect can be reached.