The invention relates to a sequencing batch type non-waste biological degumming method for ramie. The sequencing batch type non-waste biological degumming method for ramie includes pretreatment and four times of soaking. In first soaking, pretreated ramie is soaked in degumming wastewater for 8 hours, which is bleached and biologically inactivated. In second soaking, the pretreated ramie is soaked in facultative anaerobic degumming bacteria solution with 107cfu/ml live bacteria at 37 DEG C for 8 hours. In third soaking, the ramie soaked for the first time is soaked in aerobic degumming bacteria solution with 107cfu/ml live bacteria at 37 DEG C for 8 hours. In fourth soaking, the ramie soaked for the second time is soaked in solution of 5g/L EDTA-Na2 (ethylene diamine tetraacetic acid disodium) and 2g/L NaOH at 55 DEG C for 4 hours and is finally heated to 80 DEG C prior to thermal insulating for 1 hour. Bath ratio in each time of soaking is 1:(10-20). The sequencing batch type non-waste biological degumming method degums the ramie and treats degumming wastewater simultaneously, the degumming wastewater is treated and all reclaimed, and no wastewater discharges.