The purpose of the invention is to provide a method with easy operation and high accuracy for screening toxicity of traditional Chinese medicine by model organism zebra fish. The method comprises the following steps: dissolving a crushed or extracted traditional Chinese medicine material or a patented Chinese herbal prescription into water, preparing test solutions with different concentrations, selecting healthy and active zebra fish with similar sizes, and putting the zebra fish randomly into clean glass jars with an experimental temperature of (20+/-2) DEG C, continuously observing for 4 hours after the experiment begins, simultaneously carrying out untime observation within 120 hours at a frequency of at least one time observation in every 6 hours, picking out dead fish timely, when the fish fries are poisoned to stop breathing, clamping the fishtail petioles with a pincette, determining that the fish fry is dead when no response is observed in 10 min], calculating and recording accumulated mortality of the zebra fish of each group, and calculating LC 50 and 95% confidence interval by utilizing a karbers method. The method is characterized by a simple operation method and a strong practicality; the method can preliminarily determine the toxicity of the traditional Chinese medicine with a fast speed and a high accuracy.