The invention particularly relates to a pig breeding and respiratory syndrome NVDC-JXA1 strain-porcine parvovirus disease duplex inactivated vaccine and a preparation method and application thereof. The pig breeding and respiratory syndrome NVDC-JXA1 strain-porcine parvovirus disease duplex inactivated vaccine is prepared by enabling pig breeding and respiratory syndrome virus NVDC-JXA1 strain to inoculate with a Marc-145 cell, enabling a porcine parvovirus virulent strain to inoculate with an ST cell, respectively obtaining infection cell culture fluid to be inactivated by using formaldehyde solution, and conducting ultrafiltration, concentration and mixing to obtain mixed virus liquid to be mixed and emulsified with oil adjuvant. The inactivated vaccine comprises, by weight, 1-2 parts of the oil adjuvant and 1 part of mixed virus liquid aqueous phase, the inactivated vaccine is prepared by preparing pig breeding and respiratory syndrome virus liquid, porcine parvovirus liquid, an oil phase, the aqueous phase and emulsifying, and NVDC-JXA1 strain pig breeding and respiratory syndrome-porcine parvovirus disease duplex inactivated vaccine quality standards are built.