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发明公开
CN102676680A 一种鉴别Q型烟粉虱单倍型的引物及鉴别方法 失效 - 权利终止

一种鉴别Q型烟粉虱单倍型的引物及鉴别方法
摘要：
本发明涉及一种鉴别Q型烟粉虱单倍型的引物及鉴别方法，步骤如下：（1）提取Q型烟粉虱基因组DNA；（2）以Q型烟粉虱基因组DNA为模板对其线粒体COI基因进行PCR扩增；（3）对步骤（2）制得的PCR产物用限制性内切酶Hin1II(N1aIII)酶切，得酶切产物；（4）对步骤（3）制得的酶切产物进行琼脂糖凝胶电泳分析。本发明所述的限制性内切酶为常用的限制性内切酶，为筛选两种单倍型的Q型烟粉虱提供了简便稳定的酶切标记，同时探索建立了两种单倍型的Q型烟粉虱的鉴别技术，为今后的两种单倍型的Q型烟粉虱的种群动态鉴定、生物学以及入侵机制的研究奠定了基础。
摘要（英）：

The invention relates to a haplotype primer for identifying Q-shaped bemisia tabaci and an identification method. The method comprises the following steps of: (1) extracting Q-shaped bemisia tabaci genomic DNA; (2) carrying out PCR (Polymerase Chain Reaction) amplification on mitochondrial COI (cytochrome oxidase subunit I) genes of the Q-shaped bemisia tabaci genomic DNA by taking the Q-shaped bemisia tabaci genomic DNA as a template; (3) digesting a PCR product prepared in step (2) by a restriction enzyme Hin1II (N1aIII) to obtain a digestion product; and (4) carrying out agarose gel electrophoresis analysis on the digestion product prepared in step (3). The restriction enzyme is a common restriction enzyme, a simple and stable digestion mark is provided for screening two types of haplotype Q-shaped bemisia tabaci, at the same time an identification technology for the two types of haplotype Q-shaped bemisia tabaci is developed and established, and a foundation is established for future population dynamic identification and biology and invasion mechanism researches of the two types of haplotype Q-shaped bemisia tabaci.

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