The invention provides NAD (Nicotinamide Adenine Dinucleotide), an aluminum hydroxide compound adjuvant and a vaccine containing the compound adjuvant. The compound adjuvant consists of NAD and aluminum hydroxide in the mass ratio of (1-5):(50-100). The vaccine containing the compound adjuvant is obtained by adding 0.51-1.05mg of compound adjuvant into every 100 muL of antigen liquid. The compound adjuvant provided by the invention has the advantages of readily-available raw materials, simple preparation process, low cost and stability, and can be taken as an adjuvant of a hepatitis B vaccine, a protein vaccine, a virus vaccine and the like. Due to the combined application of the compound adjuvant and the vaccine, the immune response of a vaccine fluid can be enhanced effectively, and the enhancing effect is superior to those of a single NAD adjuvant and an aluminum hydroxide adjuvant; and moreover, the compound adjuvant and the vaccine are nontoxic, free from side effects and safe and reliable for using in an immune dosage range.