The invention provides a human hepatoma carcinoma cell and application thereof. The human hepatoma carcinoma cell is preserved in China Center of Typical Culture and Preservation, and the preservation number of the human hepatoma carcinoma cell is CCTCC No. C201072. The human hepatoma carcinoma cell can be used for producing human liver cancer in mammal to prepare the human liver cancer model which can be used for further selecting the candidate medicine for treating the human liver cancer. The human hepatoma carcinoma cell line has stable properties and can be transferred stably for multiple times. The properties can be maintained stable from 20th generation to 50th generation of the passages. The human hepatoma carcinoma cell line has the biological properties of the human liver cancer on the clinic, hepatitis B DNA is integrated in the cancer cell genetic group, so novel experimental materials which are more close to the biological properties of the clinic liver cancer can be provided for researching the liver cancer.