The invention discloses a cultivation method of Dendrobium nobile Lindl, wherein the bagged mixed wood dust is used as culture material; the waste resource produced by processing local wood is utilized fully; the growth environment can be regulated rationally in good time; the water, fertilizer, illumination and diseases and insect pests can be controlled in room; thus, a path of comprehensive utilization of wood resource is created; the economic income of the local farmer is increased, meanwhile the ecological economic mode of protecting trees by trees is achieved really and the local plantation level and environmental awareness of the farmer are improved.