The present invention discloses a seedling method for tissue culture and rapid breeding of camellia semiserrata chi. The method comprises: explant collecting, aseptic differentiation, strong seedling culturing and bagged seedling transplanting, wherein the explant is provided for breeding. According to the method, bud tip or leaf is adopted as the explant for the breeding, and is subjected to treatments of disinfection and impurity removing; a differentiation culture media is adopted to culture the treated bud tip or the treated leaf to form a small plant; the induced small plant is cut, and inoculated in a new differentiation culture media to carry out multiple subcultures; the resulting small plant is inoculated on the specially-made strong seedling culture media to carry out culture; finally the bagged seedling transplanting is performed. According to the present invention, the original culture media component is adopted; the culture media adopts the oriented polypropylene film bagas the culture dish; the tea seedlings breed rapidly; a lot of plant viruses can be removed, such that the original fine varietal characters can be maintained; the seedling has characteristics of good quality, high transplanting survival rate, rapid flowering, rapid fruiting, high oil yield, high oil quality and long high yield period; the method of the present invention has characteristics of strong operability, high application value and originality, and provides a new approach for the camellia oleifera seedling breeding, wherein the approach has characteristics of rapid breeding and rapid commissioning.