The invention relates to the field of genetic engineering and especially to application of the soybean flowering gene GmCOL4 in regulation and control of florescence of plants. Overexpression of GmCOL4 enables flowering of a plant (Arabidopis thaliana) to be inhibited obviously, flowering time to be delayed and a growth period to be prolonged. The invention can be used for overcoming the problem of flowering asynchronism in crossbreeding, the problem of growth period controlling of a variety of crops, vegetable, fruit and flowers, the problem of photoperiod sensitivity and the problem of introduction.