The invention relates to a mesylate cinepazide crystal form II and a preparation method thereof, which belong to the chemical pharmaceutical field. The solubility of the mesylate cinepazide crystal form II prepared through the preparation method provided by the invention is greater than that of an amorphous raw material, and thus, the mesylate cinepazide crystal form II is particularly applicableto injection agent; the chemical stability of the mesylate cinepazide crystal form II is better than that of the amorphous raw material, thus, convenience is brought for the production of a pharmaceutical, and the storage and the transportation of bulk pharmaceuticals, and meanwhile, the safety of the pharmaceutical is enhanced; and in addition, the cis isomers of the crystal form II are further and correspondingly reduced under a condition with high temperature and high humidity, so that the safety control of the pharmaceutical in clinical application is provided.