The invention relates to inorganic nano-material preparing, particularly to a preparation method for photosensitive and amphiphilic polyurethane-stabilized nano-zinc oxide, wherein a new photosensitive and amphiphilic polyurethane is adopted to carry out coating, an inverse emulsion technology is adopted to prepare the nano-zinc oxide, such that the photosensitive and amphiphilic polyurethane-stabilized nano-zinc oxide is formed. The novel nano-zinc oxide synthesized through the method provided by the present invention has a particle size of 20-800 nm, can be regulated through simply controlling reaction conditions (such as reactant concentrations, ultrasonic emulsification power and the like), and has good dispersity and good dispersion stability in an organic solvent system. The synthesis method for the photosensitive and amphiphilic polyurethane prepolymer has characteristics of simpleness, controllable process, easy availability of the raw materials, non toxicity and harm, and easy industrial production. The prepared nano-ZnO can be adopted as a functional nano-filler in various polymer systems, such that characteristics of anti-ultraviolet property, mechanical property and the like of the polymer can be improved.