The invention discloses preparation of a diploid cell attenuated live vaccine from a measles long-47 strain and a preparation process thereof. A measles attenuated live vaccine is prepared from a diploid cell strain, a virus culture vessel serves as a cell factory, and a virus solution can be collected continuously for three times and is stored in the form of concentration solution. The diploid cell attenuated live vaccine passes a monkey neurovirulence test and a safety test. The technology belongs to a great reform on measles attenuated live vaccine production, and plays a qualitative role in improving the production quality of the measles attenuated live vaccine. According to the invention, exogenous factor pollution and the possibility of allergic reaction on a few people existing in chick embryo cell (CEC) production are avoided, and the problems of low production efficiency and low quality are solved.