The invention provides an oxybutynin hydrochloride-containing self-adhesive spraying transdermal composition and a preparation method thereof. The composition comprises the following components in percentage by weight: 1 to 40 percent of active substance, 40 to 95 percent of solvent, 0.5 to 50 percent of skeleton film-forming material, 0 to 30 percent of tackifier, 0 to 30 percent of transdermal penetrating agent and 0 to 5 percent of pH regulator, wherein the active medicine comprises oxybutynin hydrochloride or tartrate, sulfate, phosphate, fumarate and basic groups thereof. The self-adhesive transdermal spray can carry medicines and control the release of the medicines, forms a uniform transparent membrane which contacts skin closely to maintain the high activity and power of long-time continuous skin penetration of the medicines in a matrix after being sprayed, and has higher bioavailability. The self-adhesive transdermal spray does not have irritation and irritability to skin, is suitable to be used for a long time, does not influence the wearing of clothes and difficultly falls off the skin.