The present invention relates to a beta-glucan-based scaffold for biological tissue engineering using radiation fusion technology, and to a production method therefor. According to the production method of the present invention for beta-glucan-based scaffold, radiation fusion tissue engineering, a beta-glucan-aqueous solution is cast and is then irradiated in a crosslinking reaction in such a way as to form a gel or solid scaffold, thereby facilitating cell attachment and making it easy to create a biomimetic environment coinductive to the growth and differentiation of stem cells. Consequently, the beta-glucan-based scaffold according to the present invention can be usefully employed as a filler for tissue regeneration, cell culturing and plastic surgery, as a filler for voids in biological tissue, as a scaffold for reconstructive and corrective plastic surgery, and for cell transplantation and drug delivery.