The invention discloses a deep processing device of ox bone paste. The deep processing device comprises a crasher and grinding equipment. The deep processing device is characterized by further comprising a sonicator, an enzymolysis tank and a high-pressure electric field sterilizing device, wherein the sonicator, the enzymolysis tank and the high-pressure electric field sterilizing device are sequentially connected; the grinding equipment comprises a roughing machine and a fine grinding machine; the crasher, the roughing machine and the fine grinding machine are sequentially connected; and the fine grinding machine is connected with the sonicator. The granularity of the ox bone paste prepared by the device is less than 3 mu m; the content of calcium ions is up to 10 mg/g; the total numberof germ is less than or equal to 50,000/g; the number of colon bacillus is less than or equal to 30/100g; and the pathogen is not detected.