The invention discloses a porcine acellular dermal matrix (PADM) pore adjusting method for tissue engineering, which is implemented by adding anion biomacromolecules or cation biomacromolecules into a sufficiently wetted PADM water solution and regulating the electrokinetic potential (zeta potential) of collagen, wherein the anion biomacromolecules are sodium polyaspartate, the cation biomacromolecules are water-soluble chitosan, and the weight ratio of the biomacromolecules to the PADM is 1:5-1:25. The method disclosed by the invention effectively adjusts the pore size of the PADM, and adjusts the structure pattern of the collagen beam; and the obtained PADM has the natural structure of collagen, which predicts that the material can have wide application prospects in repair medicine.