知搜-全球专利检索

  1. 登录
  2. 注册
发明公开
CN102245766A 采用RNP基序构建蛋白应答shRNA/RNAi控制体系 有权

采用RNP基序构建蛋白应答shRNA/RNAi控制体系
摘要：
本发明的目的是采用RNA-蛋白相互作用基序提供RNAi控制体系。本发明提供shRNA，其包括：具有与靶序列互补的序列的引导链；过客链，所述过客链与所述引导链形成双链体；和连接链，所述连接链连接所述引导链与所述过客链，其中所述连接链包括RNP衍生的蛋白结合基序序列。本发明还提供RNAi控制体系，其包括：所述shRNA；和RNP衍生的蛋白，所述RNP衍生的蛋白特异性结合到所述shRNA中的蛋白结合基序序列。
摘要（英）：

Disclosed is an RNAi regulation system utilizing an RNA-protein interaction motif. Specifically disclosed is shRNA which comprises a guide strand comprising a sequence complementary to a target sequence, a passenger strand capable of being annealed to the guide strand to form a double strand, and a linker strand capable of linking the guide strand to the passenger strand, wherein the linker strand comprises an RNP-derived protein-binding motif sequence. Also disclosed is an RNAi regulation system which comprises the shRNA and an RNP-derived protein capable of binding specifically to a protein-binding motif sequence in the shRNA.

公开/授权文献
0/0