The invention provides a method for fermentation of L-lysine, which comprises the following steps of: introducing engineering bacteria for expressing dihydrodipicolinic acid synthase variants into a first fermentation tank for culturing, and inoculating the obtained culture solution to a second fermentation tank for culturing; inoculating the obtained culture solution to a third fermentation tank for culturing according to the inoculation amount; then, continuously feeding sugar in batches to the third fermentation tank; and then, continuously feeding sugar and nitrogen sources in batches to the third fermentation tank.