The invention relates to a chemical and biological combined soil remediation method of banana wasteland and belongs to an agricultural intensive production technology. Aiming at the waste soil in which the morbidity of banana soilborne wilt in the current year is larger than 30% and the morbidity of super-serious banana soilborne wilt in the next year is larger than 40%, the method provided by the invention comprises the following steps: treating the soil by utilizing ammonium bicarbonate and lime or stronger ammonia water; killing a certain amount of soil microorganisms; controlling the number of soil fusarium oxysporum (banana wilt pathogenic bacteria) in per g of the soil to be 10<4>; applying a banana specialized-type microorganism organic fertilizer (100kg/mu); regulating the soil humidity; leading the number of externally added beneficial microorganisms in per g of soil to respectively achieve 10<6>-10<8>; and forming an advantageous group in the soil. Through treating the soil by the method, a paddy field with the current-year morbidity less than 10% has the morbidity about 30% in the next year, thus the soil with the super-various banana soilborne wilt is effectively remedied, and is reclaimed for planting bananas.