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发明公开
CN102220426A 副猪嗜血杆菌PCR检测试剂盒 失效 - 权利终止

副猪嗜血杆菌PCR检测试剂盒
摘要：
本发明公开了一种副猪嗜血杆菌PCR检测试剂盒，它包括8～12.5μl的PCRpremix缓冲液，25μl超纯水，2～3μl的MarkerDL2000，0.75～2.0μl浓度为12pmol/μl的引物2条，2μl阴性对照以及2μl阳性对照。本发明采用常规PCR法取代了费工费时的生化试验和血清学监测，研制了用于检测副猪嗜血杆菌的的PCR试剂盒，该试剂盒可以检测副猪嗜血杆菌，适用于副猪嗜血杆菌的检测、诊断和流行病学调查，能避免人为判定带来的误差，更可减少对猪造成的伤害，在24小时内就检出副猪嗜血杆菌，缩短了副猪嗜血杆菌的检出时间，具有快速、灵敏性高、特异性强等特点。
摘要（英）：

The invention discloses a PCR detection kit for hacmophilus parasuis. The kit comprises 8-12.5 microliters of PCR premix buffer, 25 microliters of ultrapure water, 2-3 microliters of Marker DL2000, 0.75-2.0 microliters of two primers with a concentration of 12pmol per microliter, 2 microliters of negative control and 2 microliters of positive control. In the invention, the conventional PCR methodis employed to replace a biochemical test and a serological surveillance which are labor and time consuming, and the PCR detection kit for detecting hacmophilus parasuis is developed. Able to detect hacmophilus parasuis, the kit is applicable to detection, diagnosis and epidemiological survey of hacmophilus parasuis. Errors caused by human judgments can be avoided, and harms on pigs are also reduced. With the kit, hacmophilus parasuis can be detected within 24 hours, which greatly shortens the detection time of hacmophilus parasuis. Thus, the kit of the invention is characterized by speediness, high sensitivity, strong specificity and the like.

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