The invention relates to the field of microbes, in particular to a sulfate reducing Citrobacter sp.strain HCSR and use thereof. The collection number of the sulfate reducing Citrobacter sp.strain HCSR is CGMCC NO.4660. The sulfate reducing Citrobacter sp.strain HCSR screened by the invention is amphimicrobe; and compared with the conventional sulfate reducing bacteria (SRB) strict anaerobic culture condition, the strain grows quickly under an aerobic condition and has a sulfate reducing characteristic under an anaerobic condition, the SO4<2-> removing capacity is improved to 6g/L from 2.85g/Lwithin 7 days, the facultatively anaerobic physiological property of the strain is fully played, the strain is easy to culture, the difficulty in treatment process is lowered, and the strain can be widely used in the control of sulfate-containing waste water.