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发明公开
CN102212528A 一种转基因水稻品系的外源插入片段的旁侧序列 无效 - 撤回

一种转基因水稻品系的外源插入片段的旁侧序列
摘要：
本发明涉及一种转基因水稻品系浙粳22Bt的外源插入片段的旁侧序列。所述旁侧序列为5’端旁侧序列及3’端旁侧序列。本发明首次克隆了转基因水稻品系浙粳22Bt的外源插入片段的旁侧序列，并且明确了转基因水稻品浙粳22Bt的外源插入片段的旁侧序列可以用作目的DNA扩增片段建立灵敏、特异性的转基因水稻品系浙粳22Bt的品系特异性定性、定量PCR检测。本发明所克隆、分离的外源插入片段的旁侧序列可以进一步用于建立转基因品系特异性PCR检测方法。
摘要（英）：

The invention relates to a flanking sequence of a foreign insert of transgene rice strain Zhe round-grained nonglutionous rice 22Bt. The flanking sequence consists of a 5'-terminal flanking sequence and 3'-terminal flanking sequence. In the invention the flanking sequence of the foreign insert of the transgene rice strain Zhe round-grained nonglutionous rice 22Bt is firstly cloned, and can be definitely used for target DNA (deoxyribonucleic acid) amplification segments to build sensitive and specific transgene rice strain Zhe round-grained nonglutionous rice 22Bt for qualitative and quantitative PCR (polymerase chain reaction) detection on strain specificity. The flanking sequence of the cloned and separated foreign insert can be further used for building a transgene strain specificity PCR detection method.

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