知搜-全球专利检索

  1. 登录
  2. 注册
发明公开
CN102212522A 一种利用RNA干扰技术防治烟粉虱的方法 失效 - 权利终止

一种利用RNA干扰技术防治烟粉虱的方法
摘要：
本发明是一种利用RNA干扰技术防治烟粉虱的方法，属于农业生物技术领域。该方法，步骤如下：(1)根据烟粉虱的基因序列选取RNAi的靶序列IAP基因，并设计靶序列的PCR扩增引物；(2)提取烟粉虱的RNA并通过反转录获得cDNA，然后PCR扩增cDNA，纯化后制得目的基因；(3)将目的基因插入到表达载体，制得含有目的基因的表达载体；(4)将含有目的基因的表达载体转化作物，获得具有防治烟粉虱的转基因作物，种植该作物即可。本发明对于利用RNAi技术研究烟粉虱的基因功能、利用RNAi技术防治烟粉虱和转基因植物防治烟粉虱具有重要的指导价值和理论意义。
摘要（英）：

The invention provides a method for preventing and treating bemisa babaci by utilizing an RNA (Ribose Nucleic Acid) interference (RNAi) technology, belonging to the technical field of agriculture and biology. The method provided by the invention comprises the following steps of: (1) selecting a target sequence IAP (Inhibitor Of Apoptosis Protein) gene of RNAi according to a gene sequence of the bemisa babaci and designing a PCR (Polymerase Chain Reaction) amplification primer of the target sequence; (2) extracting the RNA of the bemisa babaci and carrying out transcription to obtain a cDNA (Complementary Deoxyribonucleic Acid); then carrying out PCR amplification on the cDNA and purifying to prepare a target gene; (3) inserting the target gene into an expression vector to prepare the expression vector containing the target gene; (4) and transforming the expression vector containing the target gene into a crop to obtain a transgenic crop for preventing and treating bemisa babaci; and planting the crop. The method provided by the invention has important guiding value and theoretical significance on researching the gene function of the bemisa babaci by utilizing the RNAi technology, preventing and treating the bemisa babaci by utilizing the RNAi technology, and preventing and treating the bemisa babaci by utilizing the transgenic crop.

公开/授权文献
0/0