The invention discloses high-concentration furacilin micro emulsion washing fluid and a preparation method and use thereof. When used in combination of Solutol HS 15, the micro emulsion washing fluid can obtain good emulsion effect and obtain stable micro emulsion of small particle size, the surfactant film of the formed micro emulsion is more stable than that obtained by using Solutol HS 15 alone, and the furacilin can be coated in a hydrophobic area and is insusceptible to leaking into a water phase; and phospholipid and cholate, which are cosurfactants, are used, so the high-concentration furacilin micro emulsion washing fluid is low in viscosity and toxicity and therefore is suitable for administration by injection. The micro emulsion washing fluid has high stability and longer acting period; when the micro emulsion washing fluid is used, the clinic administration safety and the obedience in patients are improved, and the clinical doctor's medicine administration is more convenient; and the micro emulsion washing fluid has a reasonable price and is suitable for mass production. The micro emulsion washing fluid has high storage and transport stability; the preparation process is simple; the quality control is simple and convenient; the production cost is low; and the industrial production is convenient.