The invention discloses a sea cucumber capsule which comprises the following components and contents in parts by weight: 5-15 parts of sea cucumber enzymolysis powder, 3-8 parts of starfish enzymolysis powder, 0.6-2.4 parts of Chinese medicine extract, 0.3-1.2 parts of gingkgo extract powder and 0.3-1.2 parts of acanthopanax extract powder. The preparation method of the sea cucumber capsule comprises the following steps of: preparing powder from raw materials, such as sea cucumber, and the like through enzymolysis, Chinese medicine extraction, and the like, and then filling the powder to a capsule. According to the preparation method, nutrition components of the sea cucumber are not damaged, and through enzymolysis, macromolecular protein is changed into micromolecular polypeptide and amino acid which are easy to be absorbed by a human body. In addition, the capsule is convenient for carrying and taking.