The invention relates to a normal pulse stripping method for detecting the content of vitamin in blood samples, belonging to the technical field of vitamin analysis and detection. The method is characterized by comprising the following steps: taking blood samples to be detected, mixing the blood samples to be detected with a vitamin sample treatment solution, and detecting current signals generated when the blood samples are subjected to oxidation reduction reaction on a sensor probe of a vitamin detector by using the normal pulse stripping method; and making a standard curve through comparing the current signal values of standard samples with different vitamin concentrations, then obtaining the content of the vitamin in the blood samples to be detected according to the current signal values generated by the samples to be detected. The method provided by the invention has the advantages of high sensitivity, good accuracy, simplicity and convenience in operation, quickness, wide application range, suitability for being used in medical and health departments so as to carry out analysis and detection on vitamins A, B1, B2, B6, B9, B12, C, D, E, K1 and K3 in blood samples, and the like; and the method can be used for rapidly detecting the content of the vitamin in the blood samples.