The invention discloses a biological response system for multistage sewage treatment as well as a manufacturing method and a sewage treatment method. The biological response system is formed by connecting a multistage precipitation pretreatment unit, a multistage hydrolysis and acidizing treatment unit, an aeration oxygenation treatment unit and a multistage undercurrent wetland treatment unit through pipelines; the multistage undercurrent wetland treatment unit comprises a water inlet wetland pool, two groups of decomposition and percolation wetland pools and a water outlet wetland pool which are sequentially arrayed; and the multistage precipitation pretreatment unit, the multistage hydrolysis and acidizing treatment unit and the aeration oxygenation treatment unit are sequentially arranged on one side of the multistage undercurrent wetland treatment unit in parallel and are connected with one another through pipelines. The biological response system provided by the invention has the advantages of scientific design, reasonable structure, fewer electromechanical devices due to the adoption of an automatic flow mode, low operation cost, low sewage treatment expense, small floor area, obvious sewage treatment effect, convenience for operation and maintenance management, low cost on project capital construction and operation, and capability of carrying out purification treatment all the year round, ; the treated sewage can be directly discharged into reservoirs, rivers and lakes and is in accordance with discharge standards in China; and a bioecological technology has stronger self-regulation capability and repair capability.