The invention discloses a positioning and bone-cutting system used for minimally invasive artificial knee joint replacement, which comprises a positioning sub-system and bone-cutting sub-system, wherein the positioning sub-system comprises a plurality of reference modules and a positioning barrier module coupled with the reference modules; the bone-cutting sub-system comprises a plurality of bone-cutting drills, a plurality of bone-cutting saws, a patella trimming drill used for trimming a patella and a bone cut section trimming drill used for trimming a bone cut section; and the bone-cuttingdrills and the bone-cutting saws are limited by the positioning barrier module during the operation. In the positioning and bone-cutting system used for the minimally invasive artificial knee joint replacement, the positioning sub-system and the bone-cutting sub-system cooperate to accurately control the bone-cutting precision and the bone-cutting depth, reduce the wound area and protect the softtissues. The positioning and bone-cutting system used for the minimally invasive artificial knee joint replacement has a simple structure, is easy to use, achieves the effect of rapidly and accurately positioning and cutting the bone, and can be widely applied to minimally invasive artificial knee joint replacement.