The invention provides a retainer for storing, conveying and loading an intraocular lens (IOL). The retainer comprises the IOL, a separate type elastic fixed splint, an injection slide handle which interacts with the separate type elastic fixed splint, and a quick connector for inserting and connecting an implanting head, wherein the injection slide handle leads the IOL in the retainer to make controlled motion through an injection head which interacts with the separate type elastic fixed splint. The invention also discloses a method for loading the IOL into the implanting head by utilizing the retainer and a method for folding the IOL and directly inserting the IOL into human eyes. Secondary bacterial infection caused by implanting or loading the IOL artificially with forceps can be avoided by adopting the IOL retainer and a method for loading the IOL before surgery, and the IOL is ensured to automatically open within controllable time after the IOL is implanted into the eyes, so that the operation is simplified, the time is saved, the probability of breaking the IOL is reduced, the probability of endoophthalmitis is reduced, and the cost is reduced.