The invention relates to a traditional Chinese medicine preparation for preventing and treating fatigue due to sub-health, composed of an active component and pharmaceutically acceptable adjuvants, wherein the active component is prepared from the following crude medicines in percentage by weight: 20-50% of ginseng, 20-35% of dogwood fruit, 10-35% of cedrate and 5-15% of liquorice. The traditional Chinese medicine preparation provided by the invention can be a granular preparation, a capsule preparation or a tablet preparation, has the efficacies of invigorating the five internal organs, regulating yin and yang, supplementing qi, nourishing yin and relieving fatigue, has good curative effects on common fatigue due to sub-health in clinic, is not found having obvious toxic side effects on the heart, the liver and the gastrointestinal tracts, and is safe in use and convenient to take.