The invention belongs to the technical field of traditional Chinese medicine, and discloses a medical composition containing hirudin, hyaluronidase and hementerin and application thereof in the preparation of medicaments for treating cardiovascular diseases. The test of the anti-hypoxia ability of mice, the test of the protective action on myocardial ischemia reperfusion injury of anesthetized rats and other tests prove that the medicament prepared from hirudin, hementerin and hyaluronidase has favorable pharmacological action on cardiovascular diseases, especially cardiovascular diseases caused by coronary heart diseases, angina pectoris and endothelial cell injury.