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发明授权
CN102041265B 检测甲型H1N1病毒抗原的免疫检测试剂 有权 转让

检测甲型H1N1病毒抗原的免疫检测试剂
摘要：
本发明提供含有甲型H1N1病毒血凝集素蛋白DNA序列的重组表达载体和由其表达的针对甲型H1N1病毒抗原的DNA疫苗，将合成的甲型H1N1流感病毒HA基因插入质粒载体构建含有甲型H1N1病毒HA基因序列的重组表达载体，提取重组质粒DNA，即获得甲型H1N1病毒DNA疫苗，用甲型H1N1病毒DNA疫苗免疫小鼠，制备单克隆抗体，本发明也提供检测甲型H1N1病毒抗原的免疫学方法和免疫检测试剂，本发明获得特异性好、亲和力高的单抗细胞株和多克隆抗体，由此制备的免疫检测试剂灵敏度好、特异性高。
摘要（英）：

The invention provides an influenza A H1N1 virus hemagglutinin (HA) protein deoxyribonucleic acid (DNA) sequence-containing recombinant expression vector and a DNA vaccine expressed by the recombinant expression vector aiming at an influenza A H1N1 virus antigen. A synthesized influenza A H1N1 virus HA gene is inserted into a plasmid vector to construct the influenza A H1N1 virus HA gene sequence-containing recombinant expression vector, and recombinant plasmid DNA is extracted to prepare an influenza A H1N1 virus DNA vaccine; and the influenza A H1N1 virus DNA vaccine is used for immunizing a mouse to prepare a monoclonal antibody. The invention also provides an immunological method and an immunoassay reagent for assaying the influenza A H1N1 virus antigen. In the invention, a monoclonalantibody cell strain and a polyclonal antibody with high specificity and affinity are prepared; and the prepared immunoassay reagent has high sensitivity and high specificity.

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