The invention relates to a construction method of ganoderma spore powder polysaccharide fingerprint and a standard fingerprint of the ganoderma spore powder polysaccharide, belonging to the fingerprint technical field of the traditional Chinese medicine and products thereof and the functional food raw material and products thereof. The construction method in the invention comprises the following steps: extracting ganoderma spore powder polysaccharide; hydrolyzing a part of ganoderma polysaccharide; performing PMP derivatizing reaction on a part of the hydrolyzate of ganoderma polysaccharide; performing reversed phase HPLC fingerprint analysis and determining the standard fingerprint; performing the HPLC analysis on the polysaccharide fractions of ganoderma spore powder samples from fifteen different producing areas and comparing the fingerprints of the ganoderma spore powder samples to determine the common fingerprint characteristics so as to obtain a standard fingerprint. The fingerprint has nineteen common peaks in all, the sum of the peak areas of the common peaks accounts for 98% of the total peak area, and the peak area of each of four fingerprint peaks is more than 5% of thetotal peak area. The method in the invention is stable, has high degree of precision and good repeatability and is easy to grasp; and the quality situation and producing area of the ganoderma spore powder polysaccharide can be grasped from the overall characteristic of the chromatography, and a new scientific method for the quality control and identification of ganoderma spore powder can be provided.