The invention relates to a preparation method of a traditional Chinese medicine anti-oxidation preparation, belonging to the technical field of health-care food processing. The method comprises the following steps of: immersing parasenecio firmus powder respectively with 10 times, 8 times and 6 times of 75% ethanol for 8-10h, performing ultrasonic operation for 1h at a temperature of 30 DEG C anda power of 80W, standing, filtering, placing the filtrate in a rotary evaporator, recovering ethanol to obtain a fluid extract, adding a proper amount of water in the fluid extract and diluting into turbid liquor, then extracting respectively with 4-5 times of petroleum ether and ethyl acetate for three times, combining ethyl acetate layer extracts, decompressing and compressing the ethyl acetatelayer extracts, freezing and drying to obtain the ethyl acetate extract for later use. The product can be applied to the fields of food, medicament, and the like to develop a novel natural antioxidant, and is beneficial to industrial production.