The invention discloses a composition for treating salpingitis of birds and a preparation method thereof, aiming to provide a composition for treating the bird salpingitis, which has quick response, can address both the symptoms and root causes, reduces drug resistance rate of pathogenic bacteria and intensifies the curative effect, and a preparation method thereof. The composition consists of the following raw materials in percentage by weight: 5 to 15 percent of amoxicillin, 5 to 15 percent of metronidazole, 5 to 15 percent of aspirin, 5 percent of sodium carbonate, and the balance of anhydrous dextrose. In the composition, the amoxicillin and the metronidazole have excellent internal absorption effect and can enter the position of oviduct, can be combined to kill pathogenic bacteria causing the salpingitis of birds, and can avoid treatment failures caused by diagnosis errors; the aspirin has the functions of anti-inflammatory and diminish inflammation, can eliminate inflammation response, and improves the curing effect; and the sodium carbonate has the function of solubilizing the amoxicillin and the aspirin. Therefore, the composition can be drunk with water and is convenient to use.