The invention discloses a method for determining electron beam dose by using GPU (Graphics Processing Unit) acceleration and aims at providing an acceleration method to accelerate a method for determining electron beam dose based on a CPU (Central Processing Unit) platform on a CPU+GPU heterogeneous platform. The method for determining electron beam dose by using GPU acceleration comprises the following steps of: (1) forming an intensity matrix of an electron beam radiation field and a parameter of an accelerator electron beam; (2) selecting GPU which is used for determining the electron beam dose, distributing memory spaces for the GPU and transmitting calculating data to the GPU; and (3) using the GPU to calculate the dose of each unit of a three-dimensional dose matrix in parallel. According to the invention, the three-dimensional dose distribution of electron beams in a homogeneous phantom is determined, the calculation speed is quick, and the calculation accuracy is equivalent to the calculation accuracy of the method for determining electron beam dose distribution based on the CPU platform.