The invention provides a bacteria strain for degrading crude oil and producing rhamnolipid with high yield and application thereof, and in particular relates to a Pseudomonas aeruginosa with a preservation number of CGMCC No.4002 and a Pseudomonas aeruginosa preparation containing the same. The invention also relates to application of the Pseudomonas aeruginosa or preparation thereof to producing a biological surface-active substance by fermentation and culture, application to treating heavy metals in wastewater, application to degrading the crude oil and application to fuel scavenge and oil extraction. The Pseudomonas aeruginosa can be used for producing glycolipid biosurfactants with high yield by using waste oil as a carbon source. The biosurfactant can remarkably reduce the surface tension of a water solution, has strong emulsion capacity, effectively improves the solubility of alkane or crude oil in the water, and obviously promotes the degradation of the strain to the crude oil. The strain and fermentation liquor thereof are used for treating lead in the wastewater and have higher lead removal rate.