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发明公开
CN101915830A 一种赭曲霉毒素A荧光检测试纸条及其应用 无效 - 撤回

一种赭曲霉毒素A荧光检测试纸条及其应用
摘要：
一种赭曲霉毒素A荧光检测试纸条及其应用，涉及一种量子点标记的适配体的荧光试纸条检测赭曲霉毒素A的方法，属于荧光检测技术领域。本发明试纸条，由下吸水垫(1)，量子点偶联Aptamer①(2)，streptavidin-biotin-Aptamer②(3)，streptavidin-biotin-Aptamer③(4)，上吸水垫(5)，硝酸纤维素膜(6)和底板(7)所组成。本发明应用层析式一步竞争法原理，试纸条的上下比色带来半定量检测样品中赭曲霉毒素A残留量，在15min内，快速准确地检测出样品是否含有赭曲霉毒素A，以确定赭曲霉毒素A是否超标，能够满足食品安全对赭曲霉毒素A残留量检测需求，适用于饲料、屠宰企业及政府检测机构；本发明与现有技术相比，具有使用方便、经济快捷、制作容易、成本低廉的特点。
摘要（英）：

The invention discloses an ochratoxin A fluorescence detection test strip and application thereof, relates to a method for detecting ochratoxin A by using a fluorescence test strip of a quantum dot-labeled aptamer, and belongs to the technical field of fluorescence detection. The test strip comprises a lower water-absorbent pad (1), a quantum dot-coupled Aptamer 1 (2), a streptavidin-biotin-Aptamer 2 (3), a streptavidin-biotin-Aptamer 3 (4), an upper water-absorbent pad (5), a nitrocellulose membrane (6) and a bottom plate (7). By using a chromatography one-step competition principle, the test strip semiquantitatively detects ochratoxin A residue quantity in a semiquantitative detection sample through upper and lower colorimetric belts thereof, rapidly and accurately detects whether the sample contains the ochratoxin A within 15 min to determine whether the ochratoxin A is overproof, can meet the requirement of food safety on the detection of the ochratoxin A residue quantity, and is suitable for feeds, meat producing plants and government detection mechanisms; and compared with the prior art, the test strip has the characteristics of convenient use, economy, rapidness, simple manufacturing and low cost.

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