The invention relates to a high-vacuum pinpoint enhanced Raman device comprising an analysis room, a preparation room, a sampling room, a scanning tunnel microscope (STM) and a Raman detection preceding stage, wherein the scanning tunnel microscope is arranged in the analysis room and is connected with the Raman detection preceding stage; the analysis room, the preparation room and the sampling room are connected through sampling channels; the Raman detection preceding stage is arranged on a three-dimensional mobile platform which can move in a three-dimensional mode; an STM objective lens is arranged in an analysis cavity and is connected with a flange of a corrugated pipe connected with the Raman detection preceding stage through a collimating metal pipe; and Raman detection light paths are focused on the point of a pinpoint by the Raman detection preceding stage, the collimating metal pipe and the STM objective lens. Because the corrugated pipe is arranged in the light path, the position relation between a Raman light path and an STM device pinpoint can be regulated in a three-dimensional space by moving the three-dimensional mobile platform in order to focus lasers on the pinpoint in a Raman detecting instrument without influencing the light paths.