The invention provides a quality detection method for Chinese medicinal compound isatis root granules. The method comprises the following steps of: preliminarily estimating the quality of the Chinese medicinal compound isatis root granules by using the number and strength of characteristic fingerprint peaks in an electro-spray fingerprint of the Chinese medicinal compound isatis root granules obtained by soft ionization mass spectrometry; performing measurement by high-efficiency liquid chromatogram to obtain the uridine content of the Chinese medicinal compound isatis root granules of 0.19 to 0.22mg/g by taking uridine as a standard reference substance; and performing measurement by using ultraviolet-visible spectrophotometry and the coloring principle of a complex formed by aluminum nitrate and flavonoid compounds, and taking rutoside as the standard reference substance to obtain the rutoside-based total flavone content of the the Chinese medicinal compound isatis root granules of 1.27 to 1.30mg/g . The quality detection method for the Chinese medicinal compound isatis root granules has the advantages of qualitative and quantitative measurement and capability of ensuring scientificity and advancement.