The invention provides a seed-cultivating method of the blueberry by means of tissue cultivation and micropropagation, which comprises the following steps of: picking young sprout of the blueberry from fields in the growth season; sterilizing the surface of the young sprout under the aseptic condition; washing the sterilized young sprout with the sterile water; cutting the washed young sprout into a stem section with 1-2 bud(s); characteristically inoculating the stem section with 1-2 bud(s) on a first-generation inducting culture medium 3.0mg/L of WPM+ZT+0.02mg/L of NAA+30g/L of saccharose+6.5g/L of agar, wherein the PH value of the culture medium is 5.4; cultivating for 2-3 generations to obtain a tissue cultivation clone which is sterile and exuberantly grown and has multiple branches; using a cultivating culture medium 0.5-1.5mg/L of WPM+ZT+0.05-1.0mg/L of CPPU+30g/L of saccharose+6.5g/L of agar at the subculture and multiplication culture stage of the tissue cultivating seed of the blueberry, wherein the PH value of the culture medium is 5.4; and subculturing at a time every 50-60 days, thereby improving the propagation line by 5-10 times.