The invention discloses a production process for separating and extracting subprostrate sophora polysaccharide by an enzymatic hydrolysis method, comprising the following steps: A) adding water for soaking for 0.5h based on a mass-to-volume ratio of 1:15; B) adjusting pH value of the soak solution to be 5.0-7.0. adding papain to enable the mass volume concentration to be 1-4%, placing in water bath at the temperature of 50-60 DEG C for reacting for 120-180min, filtering, adding water in residual according to the mass-to-volume ratio of 1:15, placing in boiling water for water bath and filtering; C) merging twice filtrate, distilling and concentrating, adding 95% ethanol to enable the alcohol concentration to reach 80%, pouring supernate out, and centrifuging to obtain precipitate; D) taking precipitate, adding water therein based on the mass-to-volume ratio of 1:5 to dissolve, standing and centrifuging; E) taking supernate, adding 95% ethanol to precipitate, centrifuging, adding 95% ethanol in precipitate and stirring, centrifuging to remove ethanol to obtain the subprostrate sophora polysaccharide wet product; and F) freezing and drying to obtain a dry product. The invention can obviously improve the extraction ratio and total sugar content of the subprostrate sophora polysaccharide.