The invention belongs to the technical field of hot stamping materials, and discloses a mixed base bar code printing ribbon and a preparation method thereof, comprising a color layer. The color layer is prepared by the following materials according to weight percent: 30-45 percent of binder, 20-40 percent of wax, 20-35 percent of pigment and 2-3 percent of auxiliary agent. The preparation method of the mixed base bar code printing ribbon comprises preparation of the color layer, and the preparation process thereof comprises the following steps: weighing the binder, the wax, the pigment and the auxiliary agent of the color layer according to the proportion, diluting all the materials with dimethylbenzene, mixing all the materials uniformly, grinding, coating, and drying to form the color layer. The mixed base bar code printing ribbon has the characteristics of high-speed printing, high-definition printing, scraping resistance and wide source of printing materials.