The invention relates to oligosporol derivatives and application thereof, belonging to the technical field of microbial pesticides. A production bacterial strain Talaromyces sp. YM1-3 in the invention is collected by China General Microbiological Culture Collection Center on November 5th, 2009 with a collection number of CGMCC No.3400. In the invention, two new oligosporol metabolic products with similar structures are acquired by carrying out conventional fermentation, extraction and separation on YM1-3 and are named as oligosporol D and oligosporol E. The oligosporol metabolic products show activity on killing nematodes in an anti-nematode activity test of a liquid immersion method and both can effectively kill the nematodes with position. The oligosporol derivatives has the advantages of no pollution to the environment, low cost and good effect on killing the nematodes with position and can be applied to preparing pesticides for killing the nematodes.