The invention discloses a method for realizing frame header alignment and multi-frame zeroing, which completes header alignment processing of frame data and multi-frame zeroing processing of the frame data by an indicating signal with a 256-time single-frame cycle. The invention also discloses a device for realizing header alignment and multi-frame zeroing, which has simple realization, can effectively simplify the process of an OTN crossing dispatch system containing frame data processing, reduce complexity of system processing, and save logical resources of the system.