The invention discloses a PCR identification method for a bovine derived material in a traditional Chinese medicament, which comprises the following steps of: a), extracting DNA from the traditional Chinese medicament; b), performing PCR amplification on the DNA with a bovine derived special primer pair designed according to a satellite sequence with bovine specificity to form an amplified product; and c), performing electrophresis on the amplified product, judging whether a positive amplified strip is amplified on the DNA or not according to an electrophresis result, wherein if the positive amplified strip is amplified on the DNA, the traditional Chinese medicament is derived from a bovine animal and contains the bovine derived material, and if the positive amplified strip is not amplified on the DNA, the traditional Chinese medicament is not derived from the bovine animal and does not contain any bovine derived material. The method is simple, sensitive and rapid in operation and lowin cost, can effectively identify a bovine derived product, is particularly suitable for the identification of a deeply processed oxhide gelatin which has low DNA content and very short fragment caused by deep processing, and is suitable for massive popularization and application.