The invention relates to a precision real-time temperature test circuit, which consists of a precision voltage-stabilizing circuit, a filter and amplifier circuit and a voltage protection circuit, wherein the precision voltage-stabilizing circuit is formed by connecting a -15V power supply, a resistor R1 and a voltage stabilizing tube U1; the filter and amplifier circuit is formed by connecting a temperature sensor CN1, a resistor R2, a resistor R4, a capacitor C1 and an operational amplifier U2A; and the protection circuit is formed by connecting a +5V power supply, a resistor R3 and a diode D1. The circuit of the invention is simple in structure, reasonable in design and high in practicability and can be used in various places where precision real-time temperature test is required. The circuit has low manufacturing cost, high anti-interference capacity, highly precise test result, stable and reliable performance and wide application range.