The invention relates to a method for preparing amphiphilic macromolecule oil-displacing agent, comprising the following steps: (1) uniformly mixing acrylamide, a monomer with high steric hindrance, an amphiphilic monomer with high surface activity, and surface active agents with water, wherein the molar ratio of the acrylamide, the monomer with high steric hindrance and the amphiphilic monomer with high surface activity is 100:(0.01-10):(0.02-5); (2) adjusting the pH of the mixture obtained from the step (1) to 6.5-8.0; (3) adding initiators into mixture in the step (2) to initiate a polymerization reaction; and (4) hydrolyzing a gel obtained from the polymerization reaction of the step (3) under alkaline conditions and then drying the hydrolysis product to obtain the amphiphilic macromolecule oil-displacing agent. The monomer with high steric hindrance and the amphiphilic monomer with high surface activity effectively improve the hydrophobic effect of macromolecules and increase theoil-water interfacial activity of macromolecules; the amphiphilic macromolecule oil-displacing agent has evident tackifying effect, and has better emulsifying and solubilizing effects on crude oil.