The invention belongs to the field of veterinary traditional Chinese medicines, in particular to traditional Chinese medicine breast perfusate for treating mastitis of dairy cattle and a preparation method thereof. The traditional medicine breast perfusate comprises the following components in parts by weight: 10-40 parts of Japanese milkwort herb, 10-30 parts of suberect spatholobus stem, 10-25 parts of hypericum sampsonii hance, 10-25 parts of barbed skullcap herb, 10-20 parts of green tangerine peel, 10-40 parts of multiflorae, 10-20 parts of fructus terminaliae billericae, 10-40 parts of philippine violet herb, 10-20 parts of nutgrass galingale rhizome, 10-30 parts of raidx astragali and 10-30 parts of herba lycopi. The traditional Chinese medicine breast perfusate is a pure traditional Chinese medicine preparation prepared from traditional Chinese medicine extractive, has high content of effective component, has stronger inhibiting effect on common pathogenic bacteria such as staphylococcus aureus, escherichia coli and the like which cause mastitis of dairy cattle, and has the advantages of reasonable composition, safety, efficiency, easily obtained materials, simple preparation process, easy operation and low production cost.